The mission of the Immune Monitoring Core is to provide translational mechanistic studies in human solid organ and cellular transplantations.
The Immune Monitoring Core is located at 300 E. Superior Street, Suite 1100. It is a component of the Comprehensive Transplant Center (CTC) under the direction of Drs. Joseph Leventhal and James Mathew.
The Immune Monitoring Core is dedicated to assisting investigators with a central resource for human immune monitoring needs for translational and clinical transplantation research projects. It provides a valuable and unique research opportunity for translational mechanistic studies in organ and stem cell transplantation.
The core offers a wide variety of immune monitoring services, supported by a robust and specialized team of lab personnel and faculty directors. The core provides the necessary expertise in the increasingly specialized investigative paths within immune monitoring.
Personnel include a specialized team with associate directors in Renal, Liver, Pancreas, Islet transplantation as well as composite tissue allografts.
The Immune Monitoring Core currently provides the following services to the investigators of the CTC as well as the other investigators in Northwestern University and beyond. Investigators will be responsible for the costs incurred for their projects and prior animal protocol approval.
- Cell Cultures; Biopsy cultures
- MLR and proliferation assays by 3H-Thymidine incorporation and/or CFSE dilution,
- Treg-MLR that assays the ability of modulatory agents to induce the generation of new Tregs in culture.
- AlloSEQ Analysis - Flow sorting of MLR responding and proliferating cells (for subsequent TCR and BCR clonotypic analyses by Adaptive Biotechnologies)
- Cells Mediated Lympholysis (CML), Micro-CML and cytotoxicity assays using 51Chromium release,
- Limiting Dilution Analysis (LDA) for CTL and Helper Precursors (CTLp and HTLp),
- Colony forming assays,
- 5 & 14 Color Flow analyses for cell subsets and intracellular molecules such as FoxP3, IFN-g, etc.
- Multicolor RNAscope – an RNA ISH technique that allows for the detection of low quantities of RNA in cellular and tissue samples.
- Cytokine Assays in cell subsets (Flow) and culture supernatants (Luminex)
- ELISPOT Assays for IFN-g, Granzyme-B and other cytokines
- Transvivo Delayed Type Hypersensitivity Assay (TV-DTH).
- Analysis of HLA and Donor Specific B cells (in collaboration with FCVRRI – Translational Medicine).
- Mycoplasma testing
Starting a Project
To discuss starting a transplant project using the services of the Immune Monitoring Core of the Comprehensive Transplant Center, please contact:Hayden Polancich, MPH
Clinical Director, Immune Monitoring Core
Director, Immune Monitoring Core
Immune Monitoring Core Brochure
To read additional information about this core download our brochure.