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Histocompatibility & Engraftment Monitoring Core

At the Histocompatibility & Engraftment Monitoring Core of the Comprehensive Transplant Center, our mission it to:

  • Provide high-quality histocompatibility services to all investigators in the Feinberg School of Medicine and the larger Northwestern University research community
  • Develop state-of-the-art assays to improve selection and monitoring for solid organ and stem cell transplantation
  • Provide consultation on all aspects of histocompatibility testing in transplantation, autoimmunity, infectious diseases and allergy

The core is under the direction of David F Pinelli, PhD, F(ACHI). The core also includes two supervisors, a manager, senior and junior technologists with extensive experience in multiple tests related to solid organ and stem cell transplantation.

The Histocompatibility & Engraftment Monitoring Core is accredited by the American Society for Histocompatibility and Immunogenetics, and Tambur is certified by the American Board of Histocompatibility as a laboratory director and a clinical consultant. We are one of a handful centers within the U.S. certified by ASHI as a training facility for future HLA laboratory directors.


The Histocompatibility and Engraftment Monitoring Core currently provides the following services to the investigators of the CTC as well as other investigators in Northwestern University. Investigators will be responsible for the costs incurred for their projects. We offer a verity of high complexity laboratory assays, all performed under strict CLIA guidelines. Consultation regarding clinical interpretation of these assays is also available.
  • HLA typing for class I and class II antigens
    • low resolution by SSP
    • intermediate resolution by SSO
    • high resolution by SBT
  • HLA class I and class II antibody assessment
    • PRA analysis and cPRA calculations
    • Single Antigen Bead (SAB) assays by luminex
    • Single Antigen High Definition assays by flow cytometry
    • Complement (C1q) binding analysis
    • IgG3 subtype analysis
    • Titration studies for antibody strength assessment
  • T and B Lymphocyte flow cytometric crossmatch
    • Donor Specific
    • Surrogate for Shared Epitope analysis and other investigation
  • Engraftment monitoring with 21 genetic markers using STR analysis

Starting a Project

To discuss starting a transplant-specific project using the services of the Histocompatibility Core of the Comprehensive Transplant Center, please contact our director. We are located on the 11th floor of the Tarry Building, 300 E. Superior St.


Dave Pinelli

David Pinelli, PhD, F(ACHI)

Director, Clinical Research Laboratory and Biorepository
Assistant Director, Transplant Immunology Laboratory 

Histocompatibility & Engraftment Monitoring Core Brochure

To read additional information about this core, download the brochure.