Sanger Sequencing is the technique of choice for projects that need to obtain the nucleotide sequence of individual DNA targets, most typically vector constructs or PCR amplicons. It is required for many molecular biology applications, including molecular cloning, identification of individual disease-causing genetic variants, validation of findings from next-gen sequencing (NGS) studies, characterization of a particular genomic region, HLA typing, and genotyping of microsatellite markers.
The Sanger Sequencing Core provides access to commercial sequencing services contracted by FSM and the NU Office for Research. Samples are processed by ACGT, Inc., who are responsible for sequencing, user support and data return. The ACGT contract provides negotiated pricing and the convenience of ordering through NUcore using established payment sources.
- Premixed - template and sequencing primer mixed in the same tube prior to submission, most cost-effective
- Standard - template and sequencing primer submitted in separate tubes
- Premium - Standard with additional Sample Concentration Measurement service.
All manuscripts and grants presenting work supported by this core should include the following acknowledgement:
“This work was supported by the Northwestern University Sanger Sequencing Facility.”