Peptide Synthesis Core
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Mission
The mission of the Peptide Synthesis Core is to provide custom peptide synthesis, purification and mass spectrometry-based characterization services and technical support for members of the Simpson Querrey Institute (SQI), other Northwestern University researchers, and members of the Chicago and regional research community.
Services Offered
- Custom Peptide synthesis – automated synthesis of peptides at 0.05mmol-2mmol scales. Dye labeling and other modifications can also be carried out.
- Preparative HPLC – large scale purification of synthesized peptides based on chromatographic separation.
- Analytical HPLC – small scale purity determination of peptides and conjugates.
- Mass spectrometry:Molecular weight determination of small molecules, peptides & oligonucleotides
- LC/MS analysis for purity determination
- Pilot GMP cleanroom space available for self-use
- Lyophilization setup assistance
Equipment
- CEM Liberty Blue Microwave-Assisted Peptide Synthesizer
- Agilent 6230 Time-of-Flight (TOF) LC/MS
- Agilent 6510 Quadrupole Time-of-Flight (Q-ToF) LC/MS
- Shimadzu Prominence Preparative HPLC
- Waters Prep 150 Preparative HPLC
- Biotage V-10 Touch Solvent Evaporator
Acknowledgement
All manuscripts and grants presenting work supported by this core should include the following acknowledgement:
"[Insert Service Performed] was performed at the Peptide Synthesis Core Facility of the Simpson Querrey Institute at Northwestern University. This facility has current support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS – 2025633)."