The Keck Biophysics Facility offers access to a collection of advanced instruments for integrated studies of the structure, dynamics and interactions of biological and synthetic macro-molecules. The Keck Facility provides research groups at Northwestern with state of the art equipment, outstanding services, specialized training and technical expertise.
- Circular Dichroism Spectrometer (J-815)
- Photon-Counting Fluorescence Spectrometer (ISS PC1)
- Differential Scanning Calorimeter (VP-DSC)
- Isothermal Titration Calorimeter (ITC200)
- Surface Plasmon Resonance(Reichert 4SPR)
- HPLC (Agilent 1100)
- Size Exclusion Chromatography with Light Scattering Detectors (SEC-MALS-QELS)
- Dynamic Light Scattering Instrument (BI-9000/BI-200)
- Gel Imaging System
- Phosphoimager/Fluoroimager Scanner (Typhoon 9400)
- UV/Vis/NIR Spectrophotometer with Integrating Sphere (PE Lambda 1050)
- Fluorescence Plate Reader (Gemini EM)
- NanoDrop Fluorimeter
- Low Volume DLS (Unchained Labs pUNk)
- Preparative Micro-Ultracentrifuge (sorvall MX150)
- Assistance with experimental design, sample preparation and data analysis
- Technical expertise, written manuals, guidelines and application notes
- Staff -service options available on most instruments
- Specialized staff services for advanced biophysical characterization of:
- Proteins - solubility, oligomeric state, secondary, tertiary structure assessments
- Drug-Target Interactions - binding affinity (KD), kinetic rate constants (kon, koff) thermodynamic parameters (ΔG, ΔH, ΔS), EC50 measurements, specificity and stoichiometry assays
- Consultation and assistance
- Support letters for grant applications and research proposals
- Tours and presentations of the facility for faculty or postdoctoral candidates
All manuscripts and grants presenting work supported by this core should include the following acknowledgement:
"This work was supported by the Northwestern University Keck Biophysics Facility and a Cancer Center Support Grant (NCI CA060553)."
The Keck Biophysics Facility is located on the Evanston Campus, in Cook Hall, Rooms 4106 and 4118S.
The office is located in Cook Hall, Room 4134.
All instruments are accessible to trained users on a 24/7 basis.