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High Throughput Analysis Laboratory

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The NU-HTA provides researchers with equipment, advanced technology, and expertise for the development and execution of chemical screening for drug/biomarker discovery and high throughput (HTP) biological analysis. Instrumentation for plate based 96/384/1536 well liquid handling and assay detection available for walkup use for medium and high throughput assays.

Equipment Available

The NU-HTA features state-of-the-art laboratory automation equipment, including instruments for automated liquid handling and plate detection. All instruments are accessible to trained users on a 24/7 basis.

Services Offered

NU-HTA provides researchers access to innovative technology for large-scale screening and drug discovery. Levels of service range from walk-up instrument use to fully supported projects.

The HTA provides the following general services:
  • Drug discovery or biological screening
  • Optimization and miniaturization of HTP assays
  • Walk-up use of robotic liquid handling and detection equipment
  • Grant preparation assistance for HTP projects
  • Chemical and biological libraries
Assays performed include but are not limited to:
  • High content imaging and analysis in both confocal and widefield modes
  • Cell-based assays
    • Promoter/reporter assays (GFP, Luciferase)
    • Cell proliferation, viability, cytotoxicity
    • Ion channel and GPCR
    • Live cell/3D high content assays
  • Enzymatic assays
    • Kinase assays
    • Coupled enzyme assays
  • Protein binding assays
    • Fluorescence polarization (FP)
    • Fluorescence based thermal shift (FTS)
    • AlphaScreen/AlphaLISA
    • Nano-BiT and NanoBRET assays
  • HTP molecular biology protocolsq
    • PCR reactions in 384 well format
    • Automated DNA,RNA or protein purification
  • Large scale or complex liquid handling
    • Crystallography tray preparation
    • Tube to plate reformatting
    • Sample concentration normalization
  • Automated SPE sample prep
Available compound libraries:
  • NU-HTA manages small molecule compound libraries available for use as a screening resource.
    • ChemBridge DIVERSet-CL (50,000)
    • ChemDiv Kinase inhibitor set (20,000)
    • ChemDiv Protein-protein interaction focused set (20,000)
    • FDA-approved collection (1600)
    • Clinical collection (1800)
    • Bioactives and natural products library (2880)
    • Cell Cycle/DNA Damage library (3250)
    • Epigenetic focused library (300)


Curt Horvath, PhD
Faculty Director
(847) 491-5530

Sara Fernandez Dunne, MS
Research Lab Manager

General Contact
Tel: (847) 491-5960
Fax: (847) 467-1380


Hogan 4-140
2205 Tech Dr
Evanston, IL 60208


All manuscripts and grants presenting work supported by this core should include the following acknowledgement:

"This work was supported by the Northwestern University High Throughput Analysis Laboratory."