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Northwestern University Feinberg School of Medicine
Research

High Throughput Analysis Laboratory

Mission

The NU-HTA provides researchers with equipment, advanced technology, and expertise for the development and execution of chemical screening for drug/biomarker discovery and high throughput (HTP) biological analysis. Instrumentation for plate based 96/384/1536 well liquid handling and assay detection available for walkup use for medium and high throughput assays.

Equipment Available

The NU-HTA features state-of-the-art laboratory automation equipment, including instruments for automated liquid handling and plate detection. All instruments are accessible to trained users on a 24/7 basis.


Services Offered

NU-HTA provides researchers access to innovative technology for large-scale screening and drug discovery.  Levels of service range from walk-up instrument use to fully supported projects.

  • The HTA provides the following general services:
    • Drug discovery or biological screening
    • Optimization and miniaturization of HTP assays
    • Walk-up use of robotic liquid handling and detection equipment
    • Grant preparation assistance for HTP projects
    • Chemical and biological libraries
  • Assays performed include but are not limited to:
    • High content imaging and analysis in both confocal and widefield modes
    • Cell-based assays
      • Promoter/reporter assays (GFP, Luciferase)
      • Cell proliferation, viability, cytotoxicity
      • Ion channel and GPCR
    • Enzymatic assays
      • Kinase assays
      • Coupled enzyme assays
    • Protein binding assays
      • Fluorescence polarization (FP)
      • Fluorescence based thermal shift (FTS)
      • AlphaScreen/AlphaLISA
      • Epic label free
      • Nano-BiT and NanoBRET assays
    • HTP molecular biology protocolsq
      • PCR reactions in 384 well format
      • Automated DNA,RNA or protein purification
    • Large scale or complex liquid handling
      • Crystallography tray preparation
      • Tube to plate reformatting
      • Sample concentration normalization
    • Automated SPE sample prep
  • Available compound libraries:
  • NU-HTA manages small molecule compound libraries available for use as a screening resource.
    • ChemBridge DIVERSet-CL (50,000)
    • ChemDiv Kinase inhibitor set (20,000)
    • ChemDiv Protein-protein interaction focused set (20,000)
    • FDA-approved collection (1600)
    • Clinical collection (1800)
    • Bioactives and natural products library (2880)
    • Epigenetic focused library (300)

Acknowledgements

All manuscripts and grants presenting work supported by this core should include the following acknowledgement:

"This work was supported by the NorthwesternUniversity High Throughput Analysis Laboratory."

htal

HTAL Home Page

Order from the HTAL

Contacts

Curt Horvath, PhD  
Faculty Director
(847) 491-5530

Sara Fernandez Dunne, MS  
Research Lab Manager

General Contact
Tel: (847) 491-5960
Fax: (847) 467-1380


Location

Hogan 4-140
2205 Tech Dr
Evanston, IL 60208