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Northwestern University Feinberg School of Medicine
Research

High Throughput Analysis Laboratory

Mission

The NU-HTA provides researchers with equipment, advanced technology, and expertise for the development and execution of chemical screening for drug/biomarker discovery and high throughput (HTP) biological analysis. Instrumentation for plate based 96/384/1536 well liquid handling and assay detection available for walkup use for medium and high throughput assays.

Equipment Available

The NU-HTA features state-of-the-art laboratory automation equipment, including instruments for automated liquid handling and plate detection. All instruments are accessible to trained users on a 24/7 basis.

Services Offered

NU-HTA provides researchers access to innovative technology for large-scale screening and drug discovery.  Levels of service range from walk-up instrument use to fully supported projects.

The HTA provides the following general services:

  • Drug discovery or biological screening
  • Optimization and miniaturization of HTP assays
  • Walk-up use of robotic liquid handling and detection equipment
  • Grant preparation assistance for HTP projects
  • Chemical and biological libraries

Assays performed include but are not limited to:

  • High content imaging and analysis in both confocal and widefield modes
  • Cell-based assays
    • Promoter/reporter assays (GFP, Luciferase)
    • Cell proliferation, viability, cytotoxicity
    • Ion channel and GPCR
  • Enzymatic assays
    • Kinase assays
    • Coupled enzyme assays
  • Protein binding assays
    • Fluorescence polarization (FP)
    • Fluorescence based thermal shift (FTS)
    • AlphaScreen/AlphaLISA
    • Epic label free
    • Nano-BiT and NanoBRET assays
  • HTP molecular biology protocols
    • qPCR reactions in 384 well format
    • Automated DNA,RNA or protein purification
  • Large scale or complex liquid handling
    • Crystallography tray preparation
    • Tube to plate reformatting
    • Sample concentration normalization
  • Automated SPE sample prep

Available compound libraries:

NU-HTA manages small molecule compound libraries available for use as a screening resource.

  • ChemBridge DIVERSet-CL (50,000)
  • ChemDiv Kinase inhibitor set (20,000)
  • ChemDiv Protein-protein interaction focused set (20,000)
  • FDA-approved collection (1600)
  • Clinical collection (1800)
  • Bioactives and natural products library (2880)
  • Epigenetic focused library (300)

Acknowledgements

All manuscripts and grants presenting work supported by this core should include the following acknowledgement:

"This work was supported by the NorthwesternUniversity High Throughput Analysis Laboratory."

htal

HTAL Home Page

Order from the HTAL

Contacts

Curt Horvath, PhD  
Faculty Director
(847) 491-5530

Sara Fernandez Dunne, MS  
Research Lab Manager

General Contact
Tel: (847) 491-5960
Fax: (847) 467-1380


Location

Hogan 4-140
2205 Tech Dr
Evanston, IL 60208