The mission of the developing Metabolomics Core Facility at Robert H. Lurie Comprehensive Cancer Center of Northwestern University is to provide LC-MS based metabolomics services including identification, semi-quantification and quantification of primary and secondary metabolites from biological samples such as cultured cells, tissues and body fluids. The Metabolomics Core supports investigators engaged in basic, preclinical and clinical cancer research, including scientists examining basic mechanisms of disease, as well as those seeking to identify novel targets for therapy or biomarkers that can be used for early detection, diagnosis, prognosis or response to therapy.
- Metabolites Profiling - Comprehensive Hydrophilic Panel - Sample Run
Use LCMS to analyze sample extractions for a broad spectrum of water soluble metabolites (measure 200 plus common metabolites including amino acids, nucleotides and nucleobase/nucleosides species, TCA cycle, glycolysis, pyridine and cofactor metabolites, 2-HG, a-KG, pentose phosphate pathway, etc.). Peak areas with artificial units will be reported for semi-quantification (comparison between samples). 30-40 minute LCMS run, raw data will be provided upon request.
- Metabolites Profiling - Targeted Species - Sample Run
Use LCMS to analyze sample extractions for special water-soluble metabolites on a particular pathway. Peak areas with artificial units will be reported for semi-quantification (comparison between samples). 25-35 minute LC-MS/MS run, raw data will be provided upon request.
- Metabolites Profiling – Fluxomics - Sample Run
Use stable isotopes, such as 13C, 15N, 2H to study metabolic flux in different pathways simultaneously. Peak areas with artificial units will be reported for semi-quantification (comparison between samples). 25-35 minute LCMS run, raw data will be provided upon request.
- Metabolites Quantification - Sample Run
Use LCMS to quantify metabolites targets from sample extractions. Internal standards and standard curves will be involved. Amount or concentration will be reported. Raw data will be provided upon request.
- Method Development
Develop new assay per customer request.
- Sample Preparation
Process samples for LCMS injection.
- Data analysis
Use software to analyze LCMS data to acquire peak area of metabolites, then generate report.
Add-on charge for consultation effort above and beyond what is included in services 1-7. Charged by time (per hour). NU-HTA provides researchers access to innovative technology for large-scale screening and drug discovery. Levels of service range from walk-up instrument use to fully supported projects.
All manuscripts and grants presenting work supported by this core should include the following acknowledgement:
"Metabolomics services were performed by the Metabolomics Core Facility at Robert H. Lurie Comprehensive Cancer Center of Northwestern University."
Tel: (312) 908-8312
710 N. Fairbanks Court
Chicago, IL 60611