Metabolomics Core Facility
The mission of Metabolomics Core Facility (MCF) at Robert H. Lurie Comprehensive Cancer Center of Northwestern University is to provide: 1. LCMS based metabolomics service including identification, semi-quantification and quantification of metabolites from biological samples including but not limited to cells, bio-fluids and tissues; 2. integrative genomics service related to metabolism. MCF supports investigators engaged in basic, preclinical and clinical cancer research, including scientists examining basic mechanisms in of disease, as well as those seeking to identify novel targets for therapy or biomarkers that can be used for early detection, diagnosis, prognosis or response to therapy.
- Profiling - Comprehensive Hydrophilic Metabolites Panel (CHMP) - LCMS Run
Use LCMS to acquire signals from a broad spectrum of water soluble metabolites (250 plus targets including but not limited to amino acids, nucleotides and nucleobase/nucleosides, cofactors, Krebs cycle, TCA cycle, urea cycle, glycolysis, gluconeogenesis, pentose phosphate pathway, etc. See Target list for details). Semi-quantitative (comparison between samples) 30-40 minute run with raw data available. Fee/injection.
- Profiling - Targeted Metabolites (PTM) - LCMS Run
Use LCMS to acquire signals for specific targets-of-your-interest (hydrophilic metabolites or lipids). Semi-quantitative (comparison between samples) 25-35 minute run with raw data available. Fee/injection.
- Profiling - Fluxomics (FLUX) - LCMS Run
Use LCMS to acquire signals from specific stable isotopes labeled metabolites generated through incubation of tracers, such as 13C, 15N, 2H to study metabolic flux in specific pathways. Semi-quantitative (comparison between samples) 25-35 minute LCMS run with raw data available. Fee/injection.
- Quantification – Targeted Metabolites (QTM) - LCMS Run
Use LCMS to quantify targets-of-your-interest (hydrophilic metabolites or lipids) from sample extractions. Internal standards and standard compounds for targets are required. Fee/injection.
- Sample Preparation
Process samples for LCMS injection. Fee/hour.
- Data analysis
For profiling services, targets will be annotated, and peak areas (artificial unit) will be reported for semi-quantification (comparison between samples). For quantification services, amount or concentration will be reported. Fee/hour.
- Method Development
Develop new assay per customer request.
All manuscripts and grants presenting work supported by this core should include the following acknowledgement:
"Metabolomics services were performed by the Metabolomics Core Facility at Robert H. Lurie Comprehensive Cancer Center of Northwestern University."