Northwestern University Feinberg School of Medicine
Research

High Throughput Analysis Laboratory

Mission

The NU-HTA provides researchers with equipment, advanced technology, and expertise for the development and execution of chemical screening for drug/biomarker discovery and high throughput (HTP) biological analysis.

Equipment Available

The NU-HTA features state-of-the-art laboratory automation equipment, including instruments for automated liquid handling, plate detection, and microbial culture handling. All instruments are accessible to trained users on a 24/7 basis.

Services Offered

NU-HTA provides researchers access to cutting edge technology for large scale screening and drug discovery.  Levels of service include the full range from walk-up instrument use to fully supported projects.

The HTA provides the following general services:

  • Perform drug discovery or biological screening
  • Assist users in developing novel automated methods
  • Provide access to robotic liquid handling and detection equipment
  • Provide grant preparation assistance for HTP projects
  • Collaborate in grant application with HTP component
  • Help develop HTS ready assays to screen at local or national screening centers
  • Provide robust automated solutions
  • Provide in-house chemical and biological libraries

Assays performed include but are not limited to:

  • High content imaging analysis and screening
  • Cell-based assays
    • Promoter/reporter assays (GFP, Luciferase)
    • Cell proliferation, viability, cytotoxicity
    • Ion channel and GPCR
  • Enzymatic assays
  • Protein binding assays
    • Fluorescence polarization (FP)
    • Fluorescence based thermal shift (FTS)
    • ELISA
    • AlphaScreen/AlphaLISA
    • Epic label free
  • Whole genome or gene family RNAi screening
  • HTP molecular biology protocols
  • Micro qPCR reactions in 384 well format
  • Automated colony picking and arraying
  • Synthetic Genetic Array (SGA) analysis
  • Microbial library replication and rearraying
  • Large scale or complex liquid handling
    • Dose-response assay preparation
    • PCR plate preparation
    • Crystallography tray preparation
    • Hit picking and library reformatting
    • Tube to plate reformatting

Available in-house libraries:

Yeast Libraries:

  • Yeast TAP collection
  • Yeast GFP collection
  • Yeast GAL-GST library
  • Yeast Two Hybrid Array
  • Yeast Deletion Libraries
  • Yeast Genomic Tiling Collection

RNAi Libraries
The NU-HTA provides researchers with the latest in whole genome RNAi collections, has obtained the RNAi lentiviral libraries for both human and mouse (currently 120,000 total clones).

Compound Libraries

As a screening resource the NU-HTA houses a number of small molecule compound libraries that include over 2000 FDA approved drugs.

  • ChemDiv Structural-Diversity Set (30,000)
  • ChemBridge Drug-Like Set (20,000)
  • ASDI Library (6800)
  • NIH Clinical Collection (400)
  • Silverman Collection (custom library)
  • Spectrum Collection (2700)
  • NCI/DTP Open Chemical Repository (3200)
  • Kinase Inhibitor Collection (80)
  • Kozmin Library (7000)
  • Phosphatase Inhibitors (40)

Acknowledgements

All manuscripts and grants presenting work supported by this core should include the following acknowledgement:

"This work was supported by the NorthwesternUniversity High Throughput Analysis Laboratory."

htal

HTAL Home Page

Order from the HTAL

Contacts

Chi-Hao Luan, PhD  
Director
(847) 491-5643

Sara Fernandez Dunne, MS  
Research Technologist II

General Contact
Tel: (847) 491-5960
Fax: (847) 467-1380


Location

Hogan 4-140
2205 Tech Dr
Evanston, IL 60208