Our team works uses zebrafish to model cardiovascular diseases, including vascular malformations. We discovered that a loss of Cerebral cavernous malformation proteins causes the activation of KLF2, which is causative to endothelial defects in lowly perfused vessel beds. Together with the team of Elisabeth Tournier-Lasserve, we performed a comprehensive pharmacological screen to suppress CCM mutant phenotypes. One main focus of interest is to elucidate the impact of biomechanical signaling on the etiopathology of human vascular malformations.
Anusha SathyanarayananRead Bio
Anusha Sathyanarayanan, PhD, is a post-doctoral researcher in Seyfried Lab at the University Of Potsdam, Germany. She received her PhD in 2017 from Indian Institute of Technology Madras, India. She followed this with postdoctoral training at the Max Planck Institute for Molecular Biomedicine, Muenster, Germany, where she worked on the construction of artificial preimplantation mouse embryos until October 2020. She joined the Seyfried Lab in November 2020 and her current research focuses on understanding the role of Ephrin signaling and in the delineation of the endocardium specification program during the zebrafish heart development.
Claudia Rödel, PhDRead Bio
Claudia Rödel started her scientific career studying the mechanisms of body axis formation in Drosophila melanogaster and graduated at the Georg-August University of Göttingen in 2011. Shortly after, she started a post-doctoral fellowship in the lab of Dr. Dimitris Beis at the BRFAA in Athens Greece, who introduced her to the fascinating world of the zebrafish. With Dimitris, Claudia started a project about the influence of blood flow on the endothelial cell behavior using pharmacological manipulations and transcriptomic analyses. She realized that the zebrafish model was an immensely powerful tool to understand vascular health and disease, because it incorporates the complexity of a complete and fully functional cardiovascular system, while being a simpler vascular tree. Furthermore, the zebrafish is extremely amenable to manipulation and live imaging techniques, which allows us to simply observe endothelial cells, especially when they misbehave!
In 2015, she joined Salim Seyfried's lab as a postdoctoral fellow to further pursue her research on endothelial cell biology and started to work on a rare neurovascular disease, cerebral cavernous malformations (CCM). What fascinates her most in this disease are the triggers that are not of genetic origin, but stem from other cues such as blood flow, flow pattern and other biophysical factors.
Nastasja GrdseloffRead Bio
Nastasja is a PhD candidate in the group of Prof. Salim Seyfried at the University of Potsdam, Germany, since 2019. She is studying vascular anomalies such as CCM and HHT using the zebrafish as a disease model. Her current projects involve the roles of retinoic acid as well as nitric oxide signaling in vascular pathologies. Before starting her doctoral studies, she completed her Master‘s degree in Austria at the Institute for Molecular Biotechnology (IMBA) Vienna in the group of Dr. Sasha Mendjan. Her Master thesis focussed on in-vitro specification of epicardium from human pluripotent stem cells. Nastasja finished her Bachelor studies at the University of Veterinary Medicine in Vienna.
Van-Cuong PhamRead Bio
Van-Cuong Pham completed his Bachelor's in Hanoi (Vietnam), followed by Master's in Fukuoka (Japan). He has joined the Seyfried group at the University of Potsdam (Germany) since 2019 as a PhD candidate working under European Union’s Marie Curie project ‘V.A. Cure’, which aims to elucidate underlying mechanisms and find novel treatment for vascular anomalies diseases. As being always fascinated in using fish embryo to model human disease, he spends his time in the lab to generate transgenic and mutant fish that were later used to study the pathogenesis of vascular diseases, particularly cerebral cavernous malformation (CCM). His PhD project focuses on understanding chromatin remodeling in zebrafish CCM model by applying molecular biology and confocal imaging techniques.