The Bruker NanoWizard Sense+ is an Atomic Force Microscope that probes surface topography and investigates mechanical properties of cells such as stiffness. AFM tips can be coated with various surface chemistries which can be used to study complex biological questions including receptor ligand interaction. The NanoWizard Sense+ is eequipped with PeakForce Tapping which provided high-resolution imaging while simultaneously providing nanoscale property mapiing. This insturment also has DirectOverlay abilities allowing AFM imaging to be used in conjunction with a Nikon A1R confocal to image biological specimens. Additionally AFM can be perfomred in conjunction with widefield, TIRF and STORM microscopy.
This instrument was purchased with the generous support of the Robert H. Lurie Comprehensive Cancer Center via the Lea Charitable Trust, the Department of Cell and Developmental Biology, the Feinberg Dean's Office, and the Office for Research.
The FlexCell Tension System uses vacuum and positive air pressure to apply cyclic or static strain to cultured cells. This can mimic physiological conditions found in blood vessels, muscles, lung, etc. Preset waveforms include: static, sinusoidal, heart stimulation, triangular and square. Custom waveforms can be programmed.
The LaVision light sheet UltraMicroscopes allow users to image cleared tissue in either aqueous buffers or organic solvents.There are six light sheets for homogenous illumination and the sheet width is 4-24um. It has four available laser lines: 488, 561, 637, 785. CAM has two body options available. The first is the zoom body which features an optical zoom from 1.26x to 12.6x and a working distance of up to 6mm. The second is the Super Plan option with infinity corrected optics. There are 3 different objectives (1.1x, 4x, 12x) which allows for 0.66x to 30x imaging. The working distance ranges from 10.9mm-17mm.
The Leica DiveB Sp8 Multiphoton is an upright multiphoton tailored for deep-tissue imaging. There are two tunable lasers: a Spectra Physics Mai Tai (690-1040)nm and a Spectra Physics InSight X3 (680-1300nm). It is capable to detecting flourophores emitting from 380-780nm. There is also FLIM (fluorescent lifetime imaging) detection.
The AZ-100 covers a wide range of magnifications from 5X to 400X. It combines the advantages of stereo zoom microscopes and compound microscopes. The system can image blue, green and red-emitting fluorophores in thick tissues (e.g. Zebrafish, chicken eggs). The facility has obtained funding which will allow us to convert the system into a scanning confocal macroscope upon request. Please contact Dina for further details.
The AZ-100 was acquired through the support of Northwestern University Office for Research.
The A1 is a laser scanning confocal with four laser lines (405, 488, 561, 647). Available emission filters are 450/50, 525/50, 600/50 and 685/70. Four-color detection is possible with two standard PMTs (blue and far red) and two high-sensitivity GaAsP detectors (green and red). The system is equipped with the PerfectFocus focal drift compensation mechanism and automated XY stage.
The system was acquired through the generous support of Northwestern University Office for Research and Feinberg School of Medicine Dean’s Office. Additionally, several components of the system are on consignment from Nikon Instruments.
The A1R is a laser scanning confocal with resonant scanning technology can reach 400 fps raster scan speed. We currently have two instruments available. The first system has six laser lines (408,458,488,515,561,638). Available emission filters are 450/50, 485/40, 525/50, 545/40, 600/50 and 650LP. Four-color detection is possible with two standard PMTs (blue and far red) and two high-sensitivity GaAsP detectors (green and red) as well as multispectral detectors for accurate emission separation.
The second system has four laser lines (408, 488, 561 and 638).Emission filters available are 450/50, 525/50, 600/50 and 685/70. Four-color detection is possible with two standard PMTs (blue and far red) and two high-sensitivity GaAsP detectors (green and red) for live cell or high-sensitivity applications. Both systems are equipped with the PerfectFocus focal drift compensation mechanism, automated XY stage and an incubated Tokai Hit chamber the A1R also serves as a powerful platform for multipoint live cell imaging.
The first system was acquired through the generous support of Northwestern University Office for Research and Feinberg Dean’s Office. The second system was purchased through the support of the Lurie Cancer Center and the Feinberg Dean’s Office.
The Nikon A1R-MP is an upright mulitphoton microscope tailored for deep-tissue imaging with penetration depth of up to 1-1.5mm, depending on the sample type. The tunable Chameleon Vision titanium sapphire laser can tuned within the range of 700-1000nm for excitation of most commonly used dyes within the blue and red emission range. It has GaAsP detectors for increased sensitivity. In addition, the system is equipped with resonant scanner, capable of scanning up to ~400 frames per second, thus making this an ideal system to perform intravital imaging, even on rapid biological processes.
The A1R-MP+ multiphoton microscope was acquired through an S10 shared instrumentation grant awarded to Teng-Leong Chew (1 S10 OD010398-01).
The AXR is a laser scanning confocal with a 25mm field of view, galvano scanning up to 8192x8192 pixels, resonant scanning at 2048x2048 pixels that can scan as fast as 800 fps (small field of view). The system has four laser lines (408,488,561,640). Available emission filters are 450/50, two tunable emission band pass filters and 650LP. Four-color detection is possible with high-sensitivity GaAsP detectors. Multispectral detectors can also be performed for accurate emission separation. The system is also equipped with the PerfectFocus focal drift compensation mechanism, automated XY stage and an incubated Tokai Hit chamber. The A1R also serves as a powerful platform for multipoint live cell imaging. Additionally, the system software has AI image acquisition assistance to help users optimize capture settings and AI image denoise for higher quality images.
The system was acquired through the generous support of Northwestern University Office for Research, Feinberg Dean’s Office and the Robert H. Lurie Cancer Center.
The Nikon Biostation IMQ is an integrated cell incubator and microscopy system that allows users to conduct long-term, multi-point live cell imaging. The Biostation facilitates a broad array of time-lapse experiments by providing environmental control of temperature, humidity and gas concentration with phase and fluorescence imaging (red and green). The first system holds 35 mm glass bottom dishes, and the second system is configured with a holder for chambered coverglass.
The first system was acquired through the generous support from Northwestern University Office for Research and Skin Biology & Diseases Resources-Based Center. The second system was purchased through the support of the Northwestern University Office for Research.
The Nikon Biostation CT is a high-content live-cell imaging system featuring a 30-pate stacker, robotic arm and integrated microscopy system within a full-size cell incubator. This allows users to conduct long-term, multi-point live cell imaging in a variety of formats, up to 96-well plates. The Biostation CT facilitates a broad array of time-lapse experiments by providing environmental control of temperature, humidity and gas concentration with phase and fluorescence imaging (blue, green and red).
The Biostation CT was acquired through an S10 shared instrumentation grant awarded to Joshua Rappoport (1 S10 OD021704 01A1).
The Nikon Structured Illumination Super-Resolution Microscope realizes resolution of up to 115 nm in multiple colors (405, 488, 561, 640). We are able to capture imaging in 2D,3D (with Piezo stepping) and TIRF mode, in both fixed and in some live cells. Unlike conventional confocal microscopes, which are deblurring instruments that remove out-of-focus light through the use of pinholes, the N-SIM system utilizes moiré fringes to illuminate biological structures with spatially structured excitation light to achieve resolution beyond Ernst Abbe’s diffraction limit. Now equipped with a Tokai Hit incubated chamber, the N-SIM system is capable of live cell imaging at 37 degrees and soon will be capable of maintaining 5 percent CO2.
Purchased through a shared instrumentation grant awarded to Teng-Leong Chew by the NIH (1S10OD016342-01).
This inverted microscope supports widefield, TIRF with a 95B prime Photometrics camera. There is also Yokogawa CSU-W1 spinning disk confocal imaging with two Hamamatsu Flash 4 cameras. Those imaging modalities can be combined with a Bruker Miniscanner capable of user-defined photobleaching and photoconversion experiments. The instrument is also equipped with a Nikon H-TIRF attachment to allow for total internal reflection microscopy. Long-term live cell imaging experiment is supported by an Tokai stage CO2 incubator and Nikon Perfect Focus Ti2 microscope. Available laser lines include 405, 445, 488, 514, 561 and 647.
This inverted microscope supports widefield, TIRF, and STORM imaging. Long-term live cell imaging experiment is supported by an Tokai stage CO2 incubator and Nikon Perfect Focus Ti microscope. Available laser lines include 405, 445, 488, 514, 561 and 647.
N-STORM is able to achieve the highest resolution of all light microscopy systems within CAM. Storm takes advantage of the blinking properties of fluorescent molecules. At any given point in time, only a subset of molecules will be emitting light. A series of images are captured, allowing the signal from single fluorophores to be precisely located and reconstructed into a single image. A lateral resolution of approximately 20nm and axial of 50nm can be achieved. Please contact the staff at CAM to discuss which fluorophores are compatible with this imaging modality.
Both Ti2 systems are equipped with LED light sources and filters for imaging blue (405), green (488), red (560) and far red (640) fluorescence. The Ti2 (B) has an additional 750 excitation line for imaging Cy7 based fluorophores. Both systems are capable of RGB color capture. Both have high-sensitivity, large-format cameras that results in high-resolution images that take advantage of the larger field of view. They have a variety of objectives allowing it to capture images from cells grown on plastic tissue culture plates as well as high-resolution images from samples imaged through coverglass. The microscope also has an automated stage for precise stitching and time-lapse imaging. Our facility has a heated, CO2-regulated incubation chamber that can be added to the Ti2 microscope to image live cell experiments.
The system was acquired through the generous support of Northwestern University Office for Research, Feinberg Dean’s Office and the Lurie Cancer Center.
The Olympus DSU is a unique spinning disk microscope that comes equipped with three disks of varying slit width that giving users the capability to swap disks so that they are matched to magnification. The DSU uses arc lamp illumination for excitation wavelengths in the range of 350-700nm, allowing for imaging of reporters such as Fura dyes. The DSU also has a Tokai Hit chamber for live cell imaging as well as a Femtojet Microinjector that injects small to intermediate volumes (up to 100pl) of aqueous solutions into cells.
The Femtojet was generously donated by Sui Huang after being purchased by funding obtained by the Whitaker Fund. The Olympus DSU was acquired through the support of Northwestern University Office for Research.
The LAGO from Spectral Imaging Instruments is capable of both in vivo bioluminescent as well as fluorescent imaging of small animals. Bioluminescent in vivo imaging is an exceptionally sensitive method of tracking cell migration and growth in vivo. Fluorescence animal imaging with 14 LED wavelengths from 360-805nm and 20 emmission filters from 490-870nm and allows multiple channel acquisition. The LAGO allows imaging of up to 10 mice at time. It is housed in the pathogen free barrier in the Lurie Vivarium and accessible only by users with approved IACUC animal protocols. Users are encouraged to consult with CAM staff when designing their experiments.
The system was acquired through the generous support of Northwestern University Office for Research, Feinberg Dean’s Office and the Lurie Cancer Center.
TissueGnostics is a high-throughput, automated slide imaging system. It is capable of capturing large-format, high-resolution, stitched widefield images in either brightfield or four-color fluorescence from up to eight slides at a time. In combination with the user friendly HistoFaxs software package, scientists are able to quantify subpopulations of cells (i.e., double positive vs. total cells) and have the areas where these cells are present be highlighted in the images.
The TissueGnostics system was acquired through the support of Northwestern University Office for Research.
The PALM system uses a unique combination of a high-precision microdissection laser and a catapulting pulse of defocused laser to effectively transport the specimen of the obejct plane into a collection device. The pressure catapulting mechanism eliminates heating of thermoplastic film. Specimen are usually mounted on a poly ethylene naphthalate (PEN) or polyethylene tetraphthalate (PET) membrane. This system is also able to dissect tissue mounted glass slides coated with a PEN membrane. It is capable of cutting sections up to 200um thick with 2-3um precision. This allows for live cell imaging and microdissection of subcellular structures.
The Zeiss PALM microdissection system is purchased through a National Center for Research Resources S10 Shared Instrumentation
The FEI Tecnai Spirit G2 is a 120kV transmission electron microscope (TEM). In addition to its greatly improved capability to generate high contrast and to image much thicker samples, the system is capable of performing 3D TEM tomography. This is a tomography technique for obtaining detailed 3D structures of subcellular macromolecular object. Using a tiltable sample holder, the sample is rotated, usually at 1 degree increment during the 3D image acquisition process, as an electron beam is therefore passed through the sample. The information is then collected and used to reconstruct a three representation image of the target. Current resolutions of 3D tomography are in the 5-20 nm range, suitable for examining supra-molecular multi-protein structures, although not the secondary and tertiary structure of an individual protein or polypeptide.
It was purchased with the support of Northwestern University Office for Research. Please contact Lennell for more details or training.
The NeoScope benchtop SEM complements both optical microscopes and traditional SEMs in the lab and can be configured for advanced analytical applications. This compact electron microscope has the powerful electron optics of an SEM, with up to 60,000X magnification and offers both an Everhart Thornley type SE detector as well as high-sensitivity solid state BSE detector. The microscope has a sleek new design with up-to-date features. Operation is via a touch screen and is simplified with auto focus, auto alignment, auto contrast and auto brightness controls. The NeoScope operates in both low and high vacuum modes with three settings for accelerating voltage. These parameters are suitable for a variety of applications, all of which can be programmed in special pre-stored recipe files.
The system was acquired through the support of the Office for Research with cost share from the Feinberg Dean’s Office.
This unit can be programmed to perform stepwise, microprocessor-controlled changes in fixation conditions for user samples. It was purchased through the support of the Office for Research with cost share from the Lurie Cancer Center.
The Leica Ultracut UCT uses either a diamond or glass knife to cut ultra-thin sections of specimens. It’s ideal for biological application and soft materials preparation. Features include automatic feed in 1nm to 100 nm, cutting speed controlled to .05 mm per second, longitudinal and cross sectioning and key functions operated from the separate control unit. Please contact Lennell for more details.
This knife maker allows users to create glass knives for electron and light microscopy sectioning. The thickness of glass used is 6-7mm. Please contact Lennell for additional information.