An outstanding core facility for light and electron microscopy services.
Users who are properly trained may operate the instruments independently with the understanding that any support will be remote. Please bring a cell phone to call a CAM staff member to set up remote support session. Numbers are posted on each instrument. Data analysis support will be performed only remotely. Training sessions on most instruments are available.
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NIC Scientific Gallery
Some examples of images generated using various instruments at the Nikon Imaging Center at Northwestern University.
Combined actin, vimentin and microtubule stains in a BHK cell (Rex Chisholm's Lab)
Espin (green) bundles actin filaments (red) into microvilli that protrude upward from the monolayer of cells. (James Bartles' Lab)
Is It A Rose?
Beta4 integrin (blue) and BPAG1e (magenta) within hemisdesmosomes that have been arranged by keratinocyte into a rose-like pattern (Jonathan Jones' Lab)
Devil's Angry Eyes
This iconic picture of HeLa cells served as the promotional image for the 2011-12 One-Book-One-Northwestern event in which Rebecca Skloot's "The Immortal Life of Henrietta Lacks" was featured. (Teng-Leong Chew's Lab)
Spectrally unmixed Ki67-positive nuclei (red) within a highly autofluorescent prostate biopsy specimen. (Peter Gann's lab)
More Spiky Creatures
(James' Bartles Lab)
Ratiometric image of myosin II phosphorylation
Ptk2 cells were stained with antibody specific for phosphorylated form of myosin II regulatory light chain (RLC-p). The intensity of RLC-p stain was ratioed against GFP-RLC expressed in the cells to create this phosphorylation "heat map". (Rex Chisholm Lab)
Myosin heads along stress fibers
Comparison of myosin II sarcomeric periodicity (visualized by GFP-regulatory light chain) along stress fibers, as visualized by conventional confocal microscope (left) and N-SIM structured illumination super-resolution microscope (right). Under N-SIM, the individual globular heads of myosin II are clearly visible, as indicated by the red arrows. (Teng-Leong Chew's Lab)