Transgenic and Targeted Mutagenesis Facility Lynn Doglio, PhD, Director 312-503-0088 l-doglio@northwestern.edu

http://www.cgm.northwestern.edu/cgm/Core-Facilities/Transgenic-and-Targeted-Mutagenesis-Core

Mission Statement

The Northwestern University Transgenic and Targeted Mutagenesis Laboratory (TTML) is a shared resource designed to produce genetically engineered mice for research projects of investigators on the Evanston campus, in the Feinberg School of Medicine, the Robert H. Lurie Comprehensive Cancer Center, and Children’s Memorial Research Center (CMRC).

Services Offered

1. Generation of transgenic mice
   Transgenic mice are generated by the microinjection of purified DNA into the pronucleus of single-cell fertilized mouse embryos.  Plasmid-based or BAC DNA can be injected to create either transgenic founder lines or transgenic embryos for transient transgenesis analysis.
2. Gene targeting
   Gene targeting services, via homologous recombination in mouse embryonic stem (ES) cells, are available to investigators interested in generating genetically modified mice carrying germline targeted mutations. These services encompass the maintenance of germline competent pluripotent parental ES cell lines; all manipulation and tissue culture of ES cells necessary to create targeted clones; and microinjection of these clones into pre-implantation embryos to produce chimeric animals.
3. ES cell microinjection
   The TTML can culture and/or microinject ES cells for investigators who have either generated targeted clones in their lab or obtained ES cell clones from another source.
4. Rederivation
   The process of rederivation eliminates pathogens such as pinworm, parvovirus, and mouse hepatitis virus from infected mouse lines. Rederivation allows the import of established mouse lines with either known pathogens or of unknown health status from anywhere in the world.
5. Cryopreservation
   Cryopreservation is the process of freezing germplasm for stable, long-term storage in liquid nitrogen. It is a safe method for preserving viable pre-implantation embryos or sperm that can be easily recovered at a future date to revive the line.  Embryo and sperm cryopreservation is now available.
6. Cryorecovery
   Our cryorecovery service allows the worldwide import and recovery of mouse lines from frozen embryos or sperm.

Acknowledgements

ALL MANUSCRIPTS AND GRANTS PRESENTING WORK SUPPORTED BY THIS CORE SHOULD INCLUDE THE FOLLOWING ACKNOWLEDGEMENT

This work was supported by the Northwestern University Transgenic and Targeted Mutagenesis Laboratory and a Cancer Center Support Grant (NCI CA060553).

Location and Contacts

Lynn Doglio, PhD Director, TTML
Center for Genetic Medicine
Olson 8-402d
Phone: 312-503-0088
Fax: 312-503-0087
Email: l-doglio@northwestern.edu

Transgenic Laboratory
Lurie B-217
Phone: 312-503-0773
Fax: 312-503-0973

ES Cell Laboratory
Olson 8402
Phone: 312-503-0085
Fax: 312-503-0087

Northwestern University Feinberg School of Medicine
Research Office
Chicago, IL 60611
fsm-research@northwestern.edu
(312) 503-1499 Main
(312) 503-5603 Fax

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