http://www.cancer.northwestern.edu/research/shared_resources/mouse_histology/index.cfm The mission of the Northwestern University Mouse Histology & Phenotyping Laboratory (MHPL) is to assist investigators with gross and histological characterization of genetically modified murine models. The laboratory provides comprehensive histology services for all rodent species (mouse, rat, etc.). This includes frozen and paraffin sectioning as well as standard and special staining techniques. Histopathological analysis can be provided for single organs or they can involve a systemic overview of all major organ systems to identify histopathologic abnormalities that may exist. Pathologist consultation can also be provided to help develop strategies to elucidate phenotypes and gain mechanistic insight regarding the biologic actions of the targeted molecule or toxicity of exogenously administered substances. In addition, the laboratory provides training opportunities for learning histology techniques and phenotyping analysis. - Necropsy and tissue collection
- Gross exam with report
- Histopathology (slide interpretation)
- Toxicity evaluation in pathology
- Photo documentation of lesions
- Training in necropsy technique including perfusion of mice and special tissue dissection
- Phenotype screening (complete necropsy and histopathology of mutant mice)
- Assistance with development of animal pathology protocols
- Automated tissue processing and paraffin embedding
- Paraffin sectioning with rotary microtomes
- Serial and levels sectioning
- Standard hematoxylin & eosin staining
- Unstained slides for subsequent analysis
- Special stains e.g. Trichrome, PAS, Luxol fast blue, Cresyl violet, and many other stains
- Consultation
If a technique is not named on this page, please come see us to discuss your experiments, or send an email (MHPL@northwestern.edu) Slide Interpretation Request Form PDF
Histology Requisition PDF
Mouse Necropsy Requisition PDF
CryoJane Workshop PDF
Tissue Freezing Presentation PowerPoint
MHPL Rate Sheet Excel (netid required) ALL MANUSCRIPTS AND GRANTS PRESENTING WORK SUPPORTED BY THIS CORE SHOULD INCLUDE THE FOLLOWING ACKNOWLEDGEMENT This work was supported by the Northwestern University Mouse Histology and Phenotyping Laboratory and a Cancer Center Support Grant (NCI CA060553). Lin Li, MD. MS. ( l-li@northwestern.edu) Associate Director of MHPL
Donna Emge ASCP-HT (d-emge@northwestern.edu), Manager of MHPL
Warren Tourtellotte, M.D., Ph.D ( Warren@northwestern.edu), Director of MHPL
Olson Pavilion 8-335
710 North Fairbanks Court
Chicago, IL 60611
Tel: 312-503-2679
Fax: 312-503-2369 | 
