Launched on September 1, 2015, the NUSeq Core is a shared resource facility that provides genomics and bioinformatics support to investigators at Northwestern University, affiliated institutions, and external academic and commercial institutions. Major technologies provided include next-generation sequencing, microarray, quantitative PCR, for gene expression profiling, genotyping, epigenomics, DNA-protein interaction, 3D genome and DNA accessibility, and metagenomics. On the bioinformatics front, the Core provides data analysis and data mining services for genomics data generated in-house or externally.
Other genomic/genetic services offered by NUSeq include automated DNA extraction, cell line authentication, DNA/RNA sample measurement including QC, and Sanger sequencing (via a partnership with Chicago-based ACGT, Inc.). During the first three years of the Core’s operation, NUSeq has supported projects submitted by 1,172 researchers in 399 research groups, and generated over $3M in recharge income. While the majority of research projects accepted are internal, about 10% of Core services are requests from external institutions including Stanford, Columbia, Univ. of Chicago, Indiana Univ., Univ. of Illinois at Chicago, Loyola, Rush, NIH, etc.
The facility is the only genomics infrastructure at Northwestern, serving both Chicago and Evanston campuses. The Core’s highly trained lab and bioinformatics staff supports each project at every step of way, from experimental design to advanced data mining. Initial project consultation is provided free of charge.
- Next Generation Sequencing
- RNA-Seq (Including single-cell analysis)
- DNA-Seq (Including whole genome, exome, and gene panel sequencing)
- DNA Methyl-Seq
- Mitochondrial DNA sequencing
- Affymetrix Genechip (Gene expression)
- Illumina BeadChip (Methylome and genotyping)
- Bioinformatics and Data Mining
- Experimental Design
- NGS Data Analysis
- Microarray Data Analysis
- Advanced or Customized Data Mining
- Human Cell Line Authentication
- Automated DNA Extraction
- DNA/RNA Concentration Measurement and QC
- Traditional Sanger Sequencing
- qPCR (Equipment Use)
All manuscripts and grants presenting work supported by this core should include the following acknowledgement:
“This work was supported by the Northwestern University NUSeq Core Facility.”
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