- Tissue processing for paraffin embedded blocks (FFPE)
- Parraffin Sectioning; including specialized requests
- Frozen Specimen Sectioning
- Hematoxylin and Eosin (H&E) staining of sections
- Freezing and OCT embedding of fixed tissues
Services
The Mouse Histology & Phenotyping Laboratory (MHPL) offers extensive histology, tissue cleaning, immunohistochemistry (IHC), Immunofluorescence (IF) , TUNEL and RNA Scope (RNA in situ hybridization) services that provide consistent and standardized protocols on large and small scale projects on formalin fixed, paraffin embedded (FFPE) or frozen tissues. Sections to be stained (paraffin or frozen) should be brought to the MHPL, or they can be generated by the MHPL. Antibodies can be provided by the user or MHPL. Please contact us for an updated list of antibodies. The MHPL also provides antibody “workup” services to test novel or untried antibodies on your tissue of interest. Please contact us to arrange a consultation.
To view the rates for our services, please follow the charts on our rates list.
Available Services
- RNA Scope
- Training in necropsy techniques including perfusion of mice and special tissue dissection
- Assistance with development of animal pathology protocols
- Immunohistochemistry (IHC), Immunofluorescence (IF)
- TUNEL assay
- Custom antibody optimization for IHC/IF
- Specialized histochemical staining of sections (e.g. Trichrome, PAS, Luxol fast blue, Cresyl violet, and many other stains)
- Consultation
Histology
Below is the list of special stains for histology services, and list of antibodies for IHC, IF, and TUNEL assay services.
| Name | Classification | Level |
|---|---|---|
| Alcian Blue pH 1 | sulfated mucosubstances | 2 |
| Alcian Blue pH 2.5 | acid mucopolysaccharides | 2 |
| Bielchowsky | nerve fibers, neurofibrillary tangles, senile plaques | 3 |
| Brown and Brenn (Modified) | gram positive and gram negative bacteria | 2 |
| Cresyl Violet | Nissl body cytoplasm of Neurons | 1 |
| Fontana Masson | argentaffin (melanin) granules | 2 |
| Gordon and Sweet’s method – for reticulin | reticular fibers | 3 |
| Grocott’s Methanamine Silver Method (GMS) | fungi, pneumocystis carnii, histoplasma SPP | 3 |
| Hall’s Bilirubin stain | Bile Stain | 2 |
| Jones Methanamine Silver Method | basement membranes | 3 |
| Luxol Fast Blue | myelin | 3 |
| Luxol Fast Blue – Cresyl Violet | myelin & Nissl Substance | 3 |
| Mucicarmine (Mayer’s Method) | acidic mucins, sulphated mucins. Cryptococcus neoformans | 2 |
| Muller-mowry colloidal iron | Carboxylated, sulfated mucopolysaccharides,glycoproteins | 2 |
| Nuclear Fast Red (Kernechtrot) | counterstain | 1 |
| Oil Red O | lipids | 1 |
| PAS with Diastase Digestion | glycogen | 2 |
| Periodic Acid Schiff (PAS) | polysaccharides, basement membranes, mucosubstances, glycogen | 2 |
| Picro Sirius Red | Distinguishes collagen I from collagen III | 2 |
| Prussian Blue Reaction | iron- Ferric Form | 1 |
| Toluidine blue Acidified | mast cells | 1 |
| Trichrome- Gomoris One-Step | collagen and smooth muscle 2 | 2 |
| Trichrome - Masson's | collagen and smooth muscle | 2 |
| Verhoff-Van Geison (VVG) | elastic fibers | 2 |