The Dunbar Laboratory for Research on Memory and Fear studies the molecular and cellular mechanisms by which memories of stressful events contribute to anxiety- and depression-like behavior. Specifically, our interest lies in neuronal receptors, signal transduction pathways and gene responses mediating:
Formation and extinction of aversive memory
Delayed enhancement of fear memory in the aftermath of stressful experiences
Social modeling of fear
The relationship between aversive memories and depression
We employ molecular biological, biochemical and histological techniques to establish the cells, molecules and brain areas relevant for these processes. Pre-clinical studies with pharmacological and/or genetic mouse models are employed to establish the roles of candidate molecules in fear, anxiety and depression-like behavior. Our long-term goal is to elucidate the key brain mechanisms linking cognitive and emotional processes and identify novel targets for treatment of anxiety and depression.
Behavioral facilities equipped with: a stereotactic apparatus (Kopf Instruments), MCA microdialysis systems are for adjustable microinjection of drugs; TSE VideoMot2 activity measuring system and apparatuses used for elevated-plus maze, novelty-suppressed feeding, dark-light emergence, and open field forced tests; Two TSE fear conditioning systems for mice are for fear conditioning to context or cues.
Facilities for microscopy equipped with: confocal microscope (Olympus), microtome (Leica 3500S), fluorescent and light microscope (Leica), fluorescent Nikon inverted microscope.
- Schrick C, Fischer A, Srivastava DP, Tronson NC, Penzes P, Radulovic J. N-cadherin regulates cytoskeletally associated IQGAP1/ERK signaling and memory formation. Neuron 2007, 55:786-798.
- Sananbenesi F, Fischer A, Wang X, Schrick C, Neve R, Radulovic J, Tsai LH. A hippocampal Cdk5 pathway regulates extinction of contextual fear. Nat Neurosci 2007, 10:1012-1019.
- Tronson NC, Schrick C, Fischer A, Sananbenesi F, Pagès G, Pouysségur J, and Radulovic J. Regulatory Mechanisms of Fear Extinction and Depression-Like Behavior. Neuropsychopharmacology 2008, 33: 1570-1583.
- Tronson NC, Schrick C, Guzman YF, Huh KH, Srivastava DP, Penzes P, Guedea A, Gao C, Radulovic J. Segregated populations of hippocampal principal CA1 neurons mediating conditioning and extinction of contextual fear. J Neurosci 2009, 29: 3387-3394.
- Tronson NC, Guzman YF, Anita L. Guedea AL, Huh KH, Gao C, Schwarz MK, Radulovic J. Metabotropic Glutamate Receptor 5/Homer Interactions Underlie Stress Effects on Fear. Biol Psychiatry 2010, 68: 1007-1015.
- Gao C, Frausto SF, Guedea AL, Tronson NC, Jovasevic V, Leaderbrand K, Corcoran KA, Guzmán YF, SwansonGT, Jelena Radulovic J. IQGAP1 regulates NR2A signaling, spine density, and cognitive processes. J Neurosci 2011, 31: 8533-8542.
- Radulovic J, Tronson NC. Preso1, mGluR5, and the machinery of pain. Nat Neurosci 2012, 15:805-807.
- Corcoran KA, Donnan MD, Tronson, NC, Guzman YF, Gao C, Jovasevic V, Guedea AL, Radulovic J. NMDA receptors in retrosplenial cortex are necessary for retrieval of recent and remote contextual fear memory. J Neurosci 2011, 31: 11655-11659.
- Tronson NC, Corcoran KA, Jovasevic V, Radulovic J. Fear conditioning and extinction: emotional states encoded by distinct signaling pathways. Trends Neurosci 2012, 35:145-155.
- Guzmán YF, Tronson NC, Jovasevic V, Sato K, Guedea AL, Mizukami H, Nishimori K, Radulovic J. Fear-enhancing effects of septal oxytocin receptors. Nat Neurosci 2013 (in press)
Awards of Field Study Leadership Positions
Jelena Radulovic, MD, PhD, Dunbar Professor of Bipolar Disease, has received several honors and leadership positions throughout her career, including:
- Postdoctoral fellowship of the Max Planck Society, Dunbar Professor of Bipolar Disease, Searle Fellow, Editor (J Neuroscience)
- Senior Editor (Neurobiology of Learning and Memory), Consulting Editor (Behavioral Neuroscience), Reviewing Editor (British Journal of Pharmacology)
- Reviewer for national agencies (National Institutes of Mental Health, Department of Defense)
- Reviewer for international agencies (Welcome Trust, European Research Council, French National Research Agency, Binational Science Foundation, Israeli Science Foundation)
Grants and Funding
- National Institutes for Mental Health, NIMH R01 MH078064, R01 MH073669, Ken and Ruth Davee Award for Innovative Investigations in Affective Disorders (to Jelena Radulovic)
- CLIMB program, F31 MH092065, NISTP fellowships (to Yomayra Guzman)
- NISTP fellowships (to Katherine Leaderbrand)
- K-12 IRACDA (NU-Start)(to Kevin Corcoran)
- K99 MH093459 (to Natalie Tronson)
- NARSAD 043009 (to Can Gao)
Contact Jelena Radulovic, MD, PhD, Dunbar Professor of Bipolar Disease, for more information about the Dunbar Laboratory for Research on Memory and Fear.
Natalie Tronson, PhD
Can Gao, PhD
Cristina Schrick, Bs