Navdeep Chandel, Ph.D.
Medicine
CRISPR screen, reactive oxygen species, paraquat, redox biology
Third Floor, Feinberg Pavilion, Northwestern Memorial Hospital
Paraquat (PQ), a herbicide linked to Parkinson’s disease, generates reactive oxygen species (ROS) to cause cell death. Because the source of PQ-induced ROS production remains unknown, we conducted a CRISPR-based screen to identify genes essential for PQ-induced cell death. Our screen revealed POR (cytochrome P450 oxidoreductase) as the source of ROS essential for PQ-induced cell death and it highlights the use of functional genomic screens to uncover redox biology.