Northwestern University Feinberg School of Medicine
Research

Peptide Synthesis Core

Mission

The mission of the Peptide Synthesis Core is to provide custom peptide synthesis, purification and mass spectrometry-based characterization services and technical support for members of the Simpson Querrey Institute (SQI), other Northwestern University researchers, and members of the Chicago and regional research community.

Services Offered

  • Custom Peptide synthesis – automated synthesis of peptides at 0.1mmol-2mmol scales. Dye labeling and other modifications can also be carried out.
  • Preparative HPLC – large scale purification of synthesized peptides based on chromatographic separation.
  • Analytical HPLC – small scale purity determination of peptides and conjugates.
  • Mass spectrometry:
    • Molecular weight determination of small molecules, peptides & oligonucleotides
    • LC/MS analysis for purity determination
    • LC/MS/MS analysis for peptide sequencing
  • Lyophilization setup assistance

Equipment

  • Liberty 12-Channel Automated Microwave Peptide Synthesizer
  • CS BIO CS136XT Peptide Synthesizer
  • Agilent 6510 Quadrupole Time-of-Flight (Q-ToF) LC/MS
  • Shimadzu Prominence Preparative HPLC
  • Waters Prep 150 Preparative HPLC

Acknowledgement

All manuscripts and grants presenting work supported by this core should include the following acknowledgement:

"[Insert Service Provided] was performed in the Peptide Synthesis Core Facility of the Simpson Querrey Institute at Northwestern University. The U.S. Army Research Office, the U.S. Army Medical Research and Materiel Command, and Northwestern University provided funding to develop this facility and ongoing support is being received from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF NNCI-1542205).”

Peptide Core Home Page

Order from the Peptide Core

Contacts

Mark Karver, PhD  
Director

(312) 503-6738

Christopher Tarczynski  
Technologist
(312) 503-6725

Emily Testa  
Research Technologist
(312) 503-6725


Location

303 E. Superior St, 11-257
Phone: 312-503-6725