Northwestern University Feinberg School of Medicine
Research

Center for Advanced Microscopy

Mission

The Center for Advanced Microscopy (CAM) offers state-of-the art instrumentation and services for the study of biological processes at the whole animal, tissue, cellular, and subcellular levels. The center’s services include confocal microscopy, multiphoton imaging, super-resolution microscopy (SIM & STORM), microinjection, ratiometric calcium imaging, laser microdissection, high content imaging, whole animal bioluminescent and fluorescent imaging, transmission electron microscopy, platinum replica electron microscopy, scanning electron microscopy and 3D tomography. Many of our scopes come equipped with temperature, CO2, and humidity controlled chambers for live cell observation. We also will consult on computerized image analysis, and digital image manipulation.

Services Offered

  • Training and technical support for intravital, light and electron microscopy
  • Preparation of samples for light or electron microscopy

Equipment Available

The Center for Advanced Microscopy is located on the second floors of the adjoining Searle and Morton buildings. It consists of a 16-room suite that occupies approximately 3,950 sf. Within the facility, there are two "wet" laboratory rooms with fume hoods, one specifically for EM sample preparation, and one available for users who need immediate laboratory set-up to complete their time-dependent imaging experiments.

  • Light Microscopy
    • CRI Nuance Spectral Imaging Microscope
    • IVIS Spectrum Imaging System
    • LaVision UltraMicroscope 2 Lightsheet System
    • Nikon AZ-100 Multi-purpose Zoom Microscope
    • Nikon A1R Laser Scanning Confocal Microscopes (2)
    • Nikon A1 Laser Scanning Confocal Microscopes
    • Nikon Biostation IM-Q Live Cell Imaging Microscopes (2)
    • Nikon Biostation CT High Content Live Cell Imaging Microscope
    • Nikon C2 Laser Scanning Confocal Microscope
    • Nikon A1R-MP Multiphoton Microscope
    • Nikon N-SIM Structured Illumination Super-resolution Microscope
    • Nikon Ti2 Epi-fluorescence Microscope
    • Nikon/Yokogawa X1 Spinning Disk Confocal Microscope with STochastic Optical Reconstruction Microscopy (STORM)
    • Nikon/Yokogawa W1 Spinning Disk Confocal Microscope
    • Olympus DSU with Eppendorf Femtojet Microinjector
    • TissueGnostics Tissue Cytometer
    • Zeiss PALM Laser Catapulting Microdissection System
  • Electron Microscopy
    • Bal-Tec Med 020 Coating System
    • BOC Edwards Auto 360 coater
    • Dissecting and light microscopes
    • FEI Tecnai Spirit G2 transmission electron microscope with 3D tomography capability
    • JEOL NeoScope benchtop Scanning Electron Microscope (SEM)
    • Leica EM CPC plunge freezer
    • Leica EM Automatic Freeze Substitution System
    • Leica Ultracut UCT ultramicrotome
    • Leica Ultracut UC6 ultramicrotome
    • Leica Ultracut UC7 ultramicrotome
    • One LKB 7800 knife maker
    • Ovens for curing resin blocks
    • Tousimis Samdri 790 critical point dyer

Acknowledgement

All manuscripts and grants presenting work supported by this core should include the following acknowledgement:

"This work was supported by Northwestern University's Center for Advanced Microscopy and a Cancer Center Support Grant (NCI CA060553)."

CIF

CAM Home Page

Order from CAM

Contact

Joshua Rappoport, PhD  
Director
(312) 503-4140


Location

Searle/Mortion 2nd Floor
303 E. Chicago Avenue
Chicago, IL  60611