Northwestern University Feinberg School of Medicine
Faculty Profiles
Raj  Kishore, PhD

Raj Kishore, PhD

Adjunct Associate Professor of Medicine (Cardiology)

Focus of Work

Bio

1. Mechanisms and physiology of Interleukin-10 mediated attenuation of cardiovascular injury and inflammation. These studies have an overall focus to understand the role of inflammation in exacerbating post-injury myocardial functions and tissue repair. The strategy involves utilizing IL-10, a potent anti-inflammatory cytokine, to inhibit the expression of pro-inflammatory mediators. Past studies delineated the mechanisms by which IL-10 imparts its inhibitory action and showed that on molecular ...[Read full text]1. Mechanisms and physiology of Interleukin-10 mediated attenuation of cardiovascular injury and inflammation. These studies have an overall focus to understand the role of inflammation in exacerbating post-injury myocardial functions and tissue repair. The strategy involves utilizing IL-10, a potent anti-inflammatory cytokine, to inhibit the expression of pro-inflammatory mediators. Past studies delineated the mechanisms by which IL-10 imparts its inhibitory action and showed that on molecular level, IL-10 acts mainly via post-transcriptional de-stabilization of mRNA half-life by suppressing mRNA stabilizing protein HuR which binds to mRNAs harboring specific A+U rich sequences in their 3’ untranslated regions. Current studies are expanded utilize IL-10 and its direct downstream target HuR, as potential therapeutic approaches to inhibit inflammation in post-myocardial infarction in animal models and to elucidate signaling pathways that govern IL-10 actions both in vitro and in vivo (Rajasingh et al, FASEB J, 2006; Krishnamurthy et al, Circ Res., 2009, Krishnamurthy et al., FASEB J, 2010). 2. Epigenetic reprogramming of somatic cells to generate induced pluripotent cells and mechanisms of embryonic stem cell differentiation into cardiovascular linage cells. One component of this research focuses on to understand the molecular and signaling mechanisms governing directed differentiation of mouse embryonic stem cells into cardiomyocytes (Rajasingh J et al, Circ. Res., 2007). The second project in regenerative and stem cell biology that we have developed is to epigenetically reprogram adult somatic cells into ES-like cells (iPS cells) by utilizing cell-free ES cell protein extracts thereby bypassing the use of retroviral mediated forced expression of exogeneous transgenes (Rajasingh J et al, Circ Res., 2008). Studies are ongoing to address the mechanisms of somatic cell reprogramming and to define the role of critical epigenetic factors required for this transition.[Shorten text]

Keywords


Education and Certification

  • PhD: University of Lucknow, Immunology (1995)

Contact

312/695-4965

NMH/Arkes Family Pavilion Suite 600
676 N Saint Clair
Chicago IL 60611