Workshops

Symposium: In vivo Small Animal Imaging (November 1, 2006)
High Content Cell Analysis for Research and Drug Discovery
The Cell Imaging Facility is co-sponsoring this symposium for the NU research community. The eight seminars will be preceded by instrument demonstrations. There will be a panel discussion at the end to explore and discuss the future directions for NU animal imaging needs. Please download the PDF flyers and information through the following links.

Lunch will be served. REGISTRATION IS FREE BUT MANADATORY

Symposium Announcement

Symposium: Zeiss Cellomics HCS Readers (December 15, 2004)
High Content Cell Analysis for Research and Drug Discovery
The Cell Imaging Facility is co-sponsoring this seminar for the NU research community. Please download the PDF flyers and information through the following links.
Seminar Announcement
Detailed Product Information

Workshop: Bio-imaging in Translational Research (April 24, 2003)
Quantitative Microscopic Analysis of Human Tissue in the Digital Era

The Cell Imaging Facility is co-sponsoring a workshop to address the various technical challenges and advances in image acquisition and analysis for cancer diagnosis.

This by-invitation-only workshop will be held at:
Kellogg School of Management, Wieboldt Room 347, Chicago Campus

Topics to be covered:

Introduction: Goals of the Workshop
- Applications for image analysis in translational research
- Important methodologic issues by Peter Gann, M.D., Sc.D. (NU Dept of Preventive Medicine)

Image acquisition, Storage and Display
- slide preparation, and staining
by Michael Pins, M.D. (NU Pathology Core Facility)
- whole slide scanning: different approaches- brief overview
by Marc Key, Ph.D. (Dako Cytomation/Aperio)
- computing infrastructure requirements
by Ken Bauer, Ph.D. (Chroma Vision, Applied Imaging Corp/Ariol)

Chromogen-based Image Analysis
- Immunohistochemistry algorithms and validation
- nuclear morphometry algorithms and validation
by Jim Bacus, Ph.D.

Fluorescence-based Image Analysis
- AQUA System
by David Rimm, M.D. (Yale)
- Zeiss META "Emission Fingerprinting"
by Teng-Leong Chew, Ph.D. (NU Cell Imaging Facility)

What kind of system(s) is needed at Northwestern?
Panel Discussion

This workshop is sponsored by:
The Prostate SPORE
NU Pathology Core Facility
NU Cell Imaging Facility
Robert H. Lurie Comprehensive Cancer Center of Northwestern University

Immunogold Workshop (May 14-16, 2003)

The Cell Imaging Facility, in collaboration with Aurion Immunogold Reagents, Accessories and Custom Labeling, and Electron Microscopy Sciences will hold a three-day hands-on immunogold workshop for NU researchers. The workshop will be conducted by Dr. Jan L.M. Leunissen and Hong Yi. Dr. Leunissen is internationally recognized in the field of ultrastructural localization and has been involved in the development of ultrasmall colloidal gold conjugates. Hong Yi will be the technical coordinator for the workshop. She has a long-standing expertise in immunolocalization at the electron microscopy level. The registration fee is $400.00 per person, which will covers tuition and meals.

Main Curriculum:

The properties of gold particles and their protein conjugates.

Theories underlying immunogold labeling protocols.

Silver enhancement of gold particles.

Immunogold labeling on a variety of sample preparations for LM.

Immunogold labeling for EM
- Pre-embedding immunogold labeling using ultrasmall gold conjugates and silver enhancement.
- Post-embedding immunogold labeling using conventional colloidal gold conjugates and ultrasmall gold conjugates.

Pre- and Post-embedding double immunogold labeling.

Background minimization in immunogold labeling.

The Fryer Company will provide light microscopes for the workshop.
Please stop by the Cell Imaging Facility to collect a copy of the registration form. Users who are interested in the class must inform Mr. Lennell Reynolds by April 5, 2003, and send the application form to the proper address.

Please click here for registration form in PDF format.

Cell Imaging Workshop (Fall , 2003)

Cytoskeletal network in a fibroblast.

The Cell Imaging Facility will host a one-week hands-on workshop on fluorescent microscopy annually starting Fall of 2003. The workshop, with an enrollment of approximately 20 students, is open to all researchers in the Chicago area with priority given to Northwestern applicants. Tuition fee remains to be determined and will cost about $400-500. Please check back for further details.

Tentative topics to be covered:

Digital acquisition

Digital image processing

3-D image acquisition and processing

Fundamentals of lasers and microscopes

Confocal microscopy: Laser scanning vs. spinning disc

Live cell imaging

Fluorescent emission fingerprinting

Fluorescent recovery after photobleaching (FRAP)

Fluorescent resonant energy transfer (FRET)

Fluorescent live-time imaging microscopy (FLIP)

Image analysis

Total internal reflection fluorescent microscopy (TIRF)

This workshop is sponsored by:
Fryer Company
Nikon
Carl Zeiss, Inc.