Transmission Electron Microscopes


Actin architecture in a filopodium and its surrounding dendritic network. Picture courtesy of Gary Borisy Lab.

The Cell Imaging Facility has two transmission electron microscopes, JEOL100CX and JEOL 1220.

JEOL 100CX
This can be used for examining ultra-thin sections of a wide range of samples embedded in plastic resins or individual molecules prepared by negative stain and metal shadowing techniques. The transmission electron microscope can operate between 20KV and 100KV and has a resolution of 0.2 nm.

For easy sample exchange, this scope employs a side-entry eccentric goniometer stage capable of holding two grids simultaneously for faster and more efficient sample viewing


JEOL 1220
This advanced microprocessor-controlled microscope has a pole piece optimized for improved contrast of biological specimens. This scope can operate between 20 to 120 KV. In addition to the traditional negative film camera, this microscope is outfitted with a megapixel-resolution Kodak digital camera.



Users can get training from Mr. Lennell Reynolds. Once authrorized to use the scope, users are allowed 24-hour access to the system. Mr. Reynolds will also perform image acquisition for users, with hourly charge of tech time.