Northwestern University Cell Imaging Facility

Director    Teng-Leong Chew, Ph.D.

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Instruments:

Laser Scanning Confocal

Photo-activation & Conversion

Spinning Disc Confocal

Fluor. Emission Fingerprinting

Nuance Spectral Unmixing System

Total Internal Reflection Fluor. 

Axioskop Fluo. Microscope

Microinjector

Rotary Shadowing System

Ultramicrotomes

Trans. Electron Microscopes

Software:

MetaMorph 6.0

Volocity 2.0

Zeiss LSM 510 Software

Zeiss Image Examiner

Resources:

Publications

Links

Technical Tips

References and Books

 

 

Web Design: Teng-Leong Chew




Publications:

Please kindly send us the reference if the service provided by the Cell Imaging Facility has helped in your publication. The articles below are not listed in any particular order.

A fluorescent resonant energy transfer-based biosensor reveals transient and regional myosin light chain kinase activation in lamella and cleavage furrows.
Chew T. L., Wolf W. A., Gallagher P. J., Matsumura F., and Chisholm R. L.
Journal of Cell Biology 156(3): 543-553, 2002

Polyglutamine protein aggregates are dynamic.
Kim S., Nollen E.A., Kitagawa K., Bindokas V.P., and Morimoto R.
Nature Cell Biology 4(10): 826-31, 2002

Monitoring protein dynamics using FRET-based biosensors
Chew T. L. and Chisholm R. L.
Live Cell Imaging: A Laboratory Manual
Cold Spring Harbor Press Laboratory Press

Structure and function of a vimentin-associated matrix adhesion in endothelial cells.
Gonzales, M., Weksler, B., Hopkinson, S.B., Tsuruta, D., Goldman, R.D., Yoon, K.J., Flitney, F.W. and Jones, J.C.R.
Molecular Biology of the Cell 12: 85-100, 2001

SadA, a novel adhesion receptor in Dictyostelium
Fey, P., Stephens, S., Titus, M. A., and Chisholm R. L.
Journal of Cell Biology 159(6): 1109-1119, 2003

Interactions of a hemidesmosome component and actinin family members.
Gonzalez A.M., Edlund, M., Otey, C. and Jones, J.C.R. (2001).
Journal of Cell Science 114: 4197-4206.

Follicle stimulating hormone stimulates protein kinase A mediated histone H3 phosphorylation and acetylation leading to select gene activation in ovarian granulosa cells.
Salvador, L., Park, Y., Cottom, J., Maizels, E.T., Jones, J.C.R., Schillace, R.V., Carr, D.W., Cheung, P., Allis, C.D., Jameson, L.J. and Hunzicker-Dunn, M.
Journal of Biological Chemistry 276: 40146-40155, 2001

A requirement for cytoplasmic dynein and dynactin in intermediate filament network assembly and organization.
Helfand, B.T., Mikami, A., Vallee, R.B., and Goldman, R.D.
Journal of Cell Biology 157(5): 795-806, 2002

Microfilament-dependent movement of the ß3 integrin subunit within focal contacts of endothelial cells.
Tsuruta, D., Gonzales, M., Hopkinson, S.B., Otey, C., Khuon, S., Goldman, R.D. and Jones, J.C.R. (2002).
FASEB Journal 16: 866-868

ß4 integrin-dependent formation of polarized three-dimensional architecture confers resistance to apoptosis in normal and malignant mammary epithelium.
Weaver, V.M., Lelievre, S., Lakins, J.N., Chrenek, M.A., Jones, J.C.R., Giancotti, F., Werb, Z and Bissell, M.J.
Cancer Cell  2: 205-216, 2002

Function of laminin and laminin-binding integrins in gingival cell adhesion.
Pakkala, T., Virtanen, I., Okasnen, J., Jones, J.C.R. and Hormia, M.
Journal of Periodontology  73: 709-719, 2002

Complex interactions between the laminin a4 subunit and integrins regulate endothelial cell behavior in vitro and angiogenesis in vivo.
Gonzalez, A.M., Gonzales, M., Herron, G.S., Nagavarapu, U., Hopkinson, S.B., Tsuruta, D and Jones, J.C.R.
Proc. National Acad. Science  99: 16075-16080, 2002

Hemidesmosome protein dynamics in live epithelial cells.
Tsuruta, D., Hopkinson, S.B. and Jones, J.C.R.
Cell Motility and Cytoskeleton  54:122-134, 2003

The role of α3ß1 integrin in determining the supramolecular organization of laminin-5 in the extracellular matrix.
deHart, G.W., Healy, K.E. and Jones, J.C.R.
Experimental Cell Research  283:67-79, 2003