MetaMorph 6.0

The Cell Imaging Facility has two copies of the MetaMorph 6.0 software, one driving image acquisition on the spinning disc confocal microscope, and one as a stand-alone image analysis system, available to users free of charge. Both copies are full version of MetaMoprh.


Highlights of MetaMorph

Intensity quantification and ratio-imaging
MetaMorph can be used to compare pixel intensity, perform region statistics, volumetric measurement, morphometric studies, and ratio-imaging. If you are new to imaging, please consult the facility staff about the choice of data displays. There are times wherein direct comparison of fluorescent intensity between two images becomes less reliable, thus underscore the need to perform ratio-imaging instead. The Cell Imaging Facility is now involved in the FRET ratio-imaging software design, and will serve as one of the beta test site for the new versions of the software. We are therefore at the forefront of fluorescent image analysis, do take advantage of this unique opportunity to learn more about fluorescent imaging.



Activity and localization of myosin light chain kinase during cytokinesis, displayed by FRET ratio-imaging.
Video courtesy of Rex Chisholm's Lab

Particle Tracking
By thresholding the image, users can perform particle measurement and dynamic particle tracking to quantify the velocity of particle movement.




Thresholding allows users to quantify the number of synapsin positive particles.
Picture courtesy of Warren Tourtellotte's Lab

3-D Reconstruction/Morphometric Analysis
Users can use either copy to perform 3-D reconstruction as well as morphometric studies of Z-stacks acquired from any of the confocal microscopes in the facility.

For more information on MetaMorph, please contact Teng-Leong Chew or visit the website of Universal Imaging