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Instruments:
Laser
Scanning Confocal
Photo-activation
& Conversion
Spinning
Disc Confocal
Fluor.
Emission Fingerprinting
Nuance
Spectral Unmixing System
Total
Internal Reflection Fluor.
Axioskop Fluo. Microscope
Microinjector
Rotary Shadowing System
Ultramicrotomes
Trans. Electron Microscopes
Software:
MetaMorph
6.0
Volocity
2.0
Zeiss LSM 510
Software
Zeiss Image Examiner
Resources:
Publications
Links
Technical Tips
References
and Books
Web Design: Teng-Leong
Chew
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Gelvatol Recipe (courtesy of Goldman's Lab):
Step I
Make 100 ml solution containing 0.14M NaCl and 0.01M KH2PO4/Na2HPO4, pH 7.2
To the 100 ml solution, slowly add 25 g polyvinyl alcohol while stirring
(total volume will be more than 100ml)
Stir over night
Adjust pH to 7.2 the next day.
Step II
Add 50ml Glycerol to the solution above and stir over night (at least 16 Hours)
Spin to remove undissolved particles
Make aliquots
Store airtight, at least 4 C or colder.
Store opened vials at RT.
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