The Andor spinning disk confocal microscope is equipped with the following modules:

Yokogawa CSU-X1 spinning disk unit with adjustable spinnning speed of up to 5000rpm.

Nikon TIRF illuminator coupled with the 5-line laser launch for multi-color total internal reflection fluorescence imaging.

Nikon Ti PerfectFocus microscope stand for active compensation of thermal focal drift.

iXon3 14-bit EM CCD camera

Andor scMOS camera (with 6.5 micron pixel) for TIRF and DIC imaging

5-line, Galvo-controlled Andor FRAPPA photoablation/photomanipulation laser

Tokai-HIT stage-top CO2 incubator

The system is driven by Andor iQ2 and MetaMorph. Its configuration allows users to perform a wide spectrum of advanced live cell imaging techniques, including FRAP, FLIP, FRET, photoactivation, photoconversion, all possible in widefield, confocal and TIRF modes.

This instrument is purchased with the support of an S10 Shared Instrumentation Grant S10RR031680-01 from the National Center for Research Resources (NCRR), awarded to Dr. Vladimir Gelfand (Cell & Molecular Biology).