Nikon Imaging Center at Harvard Medical School

Equipment

Nikon C1Si Multispectral laser Scanning Confocal Microscope
The C1Si laser scanning confocal microscope is equipped with seven excitation wavelengths (402nm, 458nm, 477nm, 488nm, 514nm, 561nm, and 640nm), thus allowing users wide range of fluorophore combinations; C1Si allows users to perform not only multiplexed fluorescence imaging, but also advanced microscopy techniques such as FRET, FRAP, FLIP, photoactivation and photoconversion.

Click here for more information on C1Si.

Nikon LiveScan SFC Swept-field Confocal Microscope

The Nikon SFC confocal combines pinhole and slit scanning mode into one microscope. A hybrid system design combines tandem scanning technology with galvanometric sweeping. Apertures remain motionless (no synchronization artifact), while the galvanometric mirror sweeps an array of points across the specimen. Points are then “re-swept” to the camera to form an image. The highly efficient optical system captures a greater proportion of photons than conventional technology, with reduced back reflections from incoming light, crosstalk, and noise.

This instrument provides ultra-high scan speed of up to 100 frame per second (fps) in pinhole mode or 1000 fps in slit mode. Click here for more information.


	*Equipment* Nikon C1Si Multispectral laser Scanning Confocal Microscope The C1Si laser scanning confocal microscope is equipped with seven excitation wavelengths (402nm, 458nm, 477nm, 488nm, 514nm, 561nm, and 640nm), thus allowing users wide range of fluorophore combinations; C1Si allows users to perform not only multiplexed fluorescence imaging, but also advanced microscopy techniques such as FRET, FRAP, FLIP, photoactivation and photoconversion. Click here for more information on C1Si. Nikon LiveScan SFC Swept-field Confocal Microscope The Nikon SFC confocal combines pinhole and slit scanning mode into one microscope. A hybrid system design combines tandem scanning technology with galvanometric sweeping. Apertures remain motionless (no synchronization artifact), while the galvanometric mirror sweeps an array of points across the specimen. Points are then “re-swept” to the camera to form an image. The highly efficient optical system captures a greater proportion of photons than conventional technology, with reduced back reflections from incoming light, crosstalk, and noise. This instrument provides ultra-high scan speed of up to 100 frame per second (fps) in pinhole mode or 1000 fps in slit mode. Click here for more information.