Chicago - 1:30 PM - 6:00 PM
The Center for Genetic Medicine of Northwestern University welcomes you to attend the Mouse Genetics Group 2014 Annual Lecture & Poster Competition on May 8, featuring Allan Bradley, PhD, FRS, Director Emeritus, Wellcome Trust Sanger Institute, and Chief Scientific Officer, Kymab, Ltd. Professor Bradley will present the keynote lecture:
Full Humanization of the Mouse Immunoglobulin Loci for Therapeutic Antibody Discovery
Lecture abstract: The availability of the reference human sequence and the companion indexed BAC clone resources when combined with technologies to engineer the mouse genome provide an opportunity to humanize large regions of the mouse genome. We have applied this technology to humanize the mouse immunoglobulin heavy and light chains by precisely inserting the full complement of variable genes from the human immunoglobulin loci into the corresponding mouse genes. The mouse variable genes have been retained but they have been silenced by constructing large chromosomal inversions. Aside from the addition of 2.7 Mb of human sequence no additional DNA has been inserted or lost. Mice with these in-situ humanized loci overcome all previous limitations of transgenic models. Mice carrying these engineered chromosomes have a normal immune system and are fully viable and fertile. These mice exhibit a robust immune response to antigen challenge, producing antibodies with human-like CDR3 lengths that have strong signatures of affinity maturation. Diverse sets of antibodies can be readily isolated from these mice following antigen challenge which display broad epitope coverage and ultra-high affinities. These humanized mice provide a robust and malleable platform for therapeutic antibody discovery.
Professor Bradley currently directs the Wellcome Trust Sanger Institute mouse genomics research team, developing tools and technologies for new mouse genetics as well as exploring gene function on a large scale. Professor Bradley's team uses large-scale disruption of genes in embryonic stem (ES) cells and in somatic tissues of mice to discover genes involved in basic cellular processes as well as diseases like cancer. On a smaller scale, Dr. Bradleyâ€™s team is generating mice with mutations in individual genes to investigate their function throughout development and in adult life.
Professor Bradley received his BA, MA and PhD in Genetics from University of Cambridge. His PhD studies in the laboratory of Sir Martin Evans, FRS, winner of the Nobel Prize for Medicine 2007, laid the foundation for making knockout mice.
In 1987, Dr. Bradley accepted an appointment as an Assistant Professor at Baylor College of Medicine, Houston Texas. He was appointed a Howard Hughes Medical Institute Investigator in 1993 and was promoted to full Professor in 1994. At The Baylor College of Medicine, his laboratory played a seminal role in developing the techniques, technology and tools for genetic manipulation in the mouse. As a result, mice can now be generated with changes as subtle as an alteration in a single nucleotide or as massive as the deletion, duplication or inversion of millions of base pairs, a technology that has become known as chromosome engineering. Professor Bradley's laboratory has used ES cell technology extensively, generating and analyzing many of the first generation of mouse knockouts as well as helping numerous other laboratories to utilize this technology. This work has provided key functional information on many genes with an emphasis on cancer, DNA repair and embryonic development.
In 2000, Dr. Bradley became Director of the Sanger Centre, now called the Wellcome Trust Sanger Institute, a position he held for 10 years. In 2002, he was elected Fellow of the Royal Society. Dr. Bradley is the author of over 280 scientific articles and book chapters. Recently, Dr. Bradley founded Kymab, Ltd., a spinout company to employ contemporary mouse genetics technology for developing the next generation of therapeutic antibodies.
Abstract Submission Deadline: Friday, May 2, 2014
Northwestern University graduate students and postdoctoral fellows are invited to submit posters for the competition. Research in any field that directly involves mouse genetics is welcome.
Download a poster abstract submission form. Please complete all sections of the form and submit via email to Michelle Mohney. The submission deadline is May 2, 2014.
Cash prizes will be awarded to the top two posters presented by a graduate student or postdoc. Judging will be based on scientific merit, presentation, and creativity. Questions? Please contact Michelle Mohney atÂ firstname.lastname@example.org or 312-503-5602.
The Mouse Genetics Group Annual Lecture & Poster Competition is co-sponsored by Northwestern University's Driskill Graduate Program (DGP) in Life Sciences.